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Interferon gamma IFN γ IFNG is a dimerized soluble cytokine that is the only member of the type II class of interferon This interferon was originally called macrophage activating factor a term now used to
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Mouse Monoclonal gamma ENaC antibody
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Image Search Results
Journal: Oncogene
Article Title: Cytoplasmic interaction of the tumour suppressor protein hSNF5 with dynamin-2 controls endocytosis.
doi: 10.1038/onc.2013.276
Figure Lengend Snippet: Figure 8. Depletion of hSNF5 affects the stability of DNM2. (a) Depletion of hSNF5 or DNM2 did not affect mRNA levels of DNM2 or hSNF5, respectively. Data are average±s.d. of three independent experiments. Statistical significance was analysed by the Student’s t-test: *Po0.005. (b) Depletion of hSNF5 affects the stability of DNM2. Nuclear and cytoplasmic fractions were analysed by western blot. HSP70 and GAPDH were determined as loading controls. (c) Depletion of hBRM or/and BRG1 does not affect the stability of DNM2. Whole-cell extracts were analysed by western blot. HSP70 and GAPDH were determined as loading controls.
Article Snippet: Transient transfections were performed with FuGENE (Promega, Madison, WI, USA) or Lipofectamine 2000 (Life Technologies, Paisley, UK) for 15–36 h, as indicated by the manufacturer. small-interfering RNA oligonucleotides (siRNAs) were transfected using Oligofectamine (Life Technologies) for 72 h. Antibodies, reagents and siRNAs The following rabbit polyclonal (RpAb), goat polyconal (GpAb), mouse monoclonal (MmAb), mouse polyclonal (MpAb) and rat monoclonal (RTmAb) antibodies were used: BRG1 RpAb (sc-10768), Dynamin II GpAb (sc-6400), hSNF5 GpAb (sc-16189),
Techniques: Western Blot